Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1990 Apr;69(4):558–563.

A monoclonal antibody to Ly-6 gene product inhibits generation of functionally active T cells and recognizes single antigenic specificity whose expression is up-regulated in virus-transformed rat fibroblast.

A Haque 1, M Chamekh 1, J Cornelis 1, A Capron 1, S Haque 1
PMCID: PMC1385629  PMID: 1692303

Abstract

In order to elucidate the relationship between the structure and function of proteins encoded for by the Ly-6 gene complex, a cDNA was constructed for a Ly-6.2 specificity and then monoclonal antibodies (mAb) generated to bacterially synthesized protein. The addition of one of these mAb, designated Pb-19, inhibited the proliferative response of T cells to concanavalin A (Con A) or major histocompatability complex (MHC) alloantigens. Reactivity of Pb-19 to the Ly-6 specificity was blocked by a known anti-Ly-6.A.2 mAb but not by an anti-Ly-6.E.1 mAb. This mAb detected a Ly-6.A.2 specificity (a 33,000 MW antigen) whose expression was increased in a transformed rat fibroblast containing the entire genome of bovine papillomavirus.

Full text

PDF
561

Images in this article

560Figure 1
on p.560
561Figure 2
on p.561
562Figure 3
on p.562

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  2. Collins W. E., Anders R. F., Pappaioanou M., Campbell G. H., Brown G. V., Kemp D. J., Coppel R. L., Skinner J. C., Andrysiak P. M., Favaloro J. M. Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparum. Nature. 1986 Sep 18;323(6085):259–262. doi: 10.1038/323259a0. [DOI] [PubMed] [Google Scholar]
  3. DeLeo A. B., Shiku H., Takahashi T., John M., Old L. J. Cell surface antigens of chemically induced sarcomas of the mouse. I. Murine leukemia virus-related antigens and alloantigens on cultured fibroblasts and sarcoma cells: description of a unique antigen on BALB/c Meth A sarcoma. J Exp Med. 1977 Sep 1;146(3):720–734. doi: 10.1084/jem.146.3.720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dumont F. J. Treatment of resting T lymphocytes with interferon-alpha/beta augments their proliferative response to activation signals delivered through their surface Ly-6 antigen. Cell Immunol. 1986 Sep;101(2):625–632. doi: 10.1016/0008-8749(86)90172-3. [DOI] [PubMed] [Google Scholar]
  5. Flood P. M., Murphy D. B., Horowitz M., LeClair K. P., Smith F. R., Stockert E., Palladino M. A., Jr, DeLeo A. B. A monoclonal antibody that recognizes an Ly-6-linked antigen inhibits the generation of functionally active T cell subsets. J Immunol. 1985 Jul;135(1):63–72. [PubMed] [Google Scholar]
  6. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  7. Grisoni M., Meneguzzi G., de Lapeyrière O., Binétruy B., Rassoulzadegan M., Cuzin F. The transformed phenotype in culture and tumorigenicity of Fischer rat fibroblast cells (FR3T3) transformed with bovine papilloma virus type 1. Virology. 1984 Jun;135(2):406–416. doi: 10.1016/0042-6822(84)90196-x. [DOI] [PubMed] [Google Scholar]
  8. Gubler U., Hoffman B. J. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. doi: 10.1016/0378-1119(83)90230-5. [DOI] [PubMed] [Google Scholar]
  9. Houlden B. A., Hogarth P. M., McKenzie I. F. Interrelationships of the "Ly-6 complex" antigens. Immunogenetics. 1986;23(4):226–232. doi: 10.1007/BF00373017. [DOI] [PubMed] [Google Scholar]
  10. Korty P. E., Cohen D. I., Shevach E. M. A single cDNA encodes multiple Ly-6 antigenic specificities. Eur J Immunol. 1988 Oct;18(10):1631–1634. doi: 10.1002/eji.1830181025. [DOI] [PubMed] [Google Scholar]
  11. LeClair K. P., Palfree R. G., Flood P. M., Hammerling U., Bothwell A. Isolation of a murine Ly-6 cDNA reveals a new multigene family. EMBO J. 1986 Dec 1;5(12):3227–3234. doi: 10.1002/j.1460-2075.1986.tb04633.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lu L., Palladino M. A., Jr, DeLeo A. B. The immunotherapeutic effects in tumor-bearing mice of Ly-6 monoclonal antibodies. J Immunol. 1989 Jan 15;142(2):719–725. [PubMed] [Google Scholar]
  13. Malek T. R., Ortega G., Chan C., Kroczek R. A., Shevach E. M. Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies. J Exp Med. 1986 Sep 1;164(3):709–722. doi: 10.1084/jem.164.3.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Matossian-Rogers A., Rogers P., Ledbetter J. A., Herzenberg L. A. Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6. Immunogenetics. 1982;15(6):591–599. doi: 10.1007/BF00347053. [DOI] [PubMed] [Google Scholar]
  15. Ortega G., Korty P. E., Shevach E. M., Malek T. R. Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity. J Immunol. 1986 Nov 15;137(10):3240–3246. [PubMed] [Google Scholar]
  16. Palfree R. G., Dumont F. J., Hammerling U. Ly-6A.2 and Ly-6E.1 molecules are antithetical and identical to MALA-1. Immunogenetics. 1986;23(3):197–207. doi: 10.1007/BF00373821. [DOI] [PubMed] [Google Scholar]
  17. Palfree R. G., LeClair K. P., Bothwell A., Hämmerling U. cDNA characterization of an Ly-6.2 gene expressed in BW5147 tumor cells. Immunogenetics. 1987;26(6):389–391. doi: 10.1007/BF00343712. [DOI] [PubMed] [Google Scholar]
  18. Palfree R. G., Sirlin S., Dumont F. J., Hämmerling U. N-terminal and cDNA characterization of murine lymphocyte antigen Ly-6C.2. J Immunol. 1988 Jan 1;140(1):305–310. [PubMed] [Google Scholar]
  19. Seif R., Cuzin F. Temperature-sensitive growth regulation in one type of transformed rat cells induced by the tsa mutant of polyoma virus. J Virol. 1977 Dec;24(3):721–728. doi: 10.1128/jvi.24.3.721-728.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sutton V. R., Mickelson C. A., Tobias G. H., Hogarth P. M., McKenzie I. F. Identification of two forms of the Ly-6.2 antigen showing differential expression. Immunogenetics. 1985;21(6):539–547. doi: 10.1007/BF00395878. [DOI] [PubMed] [Google Scholar]
  21. Yeh E. T., Reiser H., Daley J., Rock K. L. Stimulation of T cells via the TAP molecule, a member in a family of activating proteins encoded in the Ly-6 locus. J Immunol. 1987 Jan 1;138(1):91–97. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES