Fig 2.

Histograms of MT-gliding velocities of the WT and L11 mutant kinesins. The assay chambers were coated with the WT kinesin (a), the wild-type and L11/L11 homodimers mixed in a proportion of 1:1 (b), L11/L11 homodimers (c), and WT/L11 heterodimers (d). The gliding velocity of the L11/L11 was much lower than the WT, but the copresence of L11/L11 did not interfere with the motility of MTs on the WT kinesin homodimers. The gliding velocity of WT/L11 was only slightly higher than that of L11/L11 homodimers. These assays were performed at 30 ± 1°C, so that the velocities were higher than the values obtained at 25 ± 1°C, as in Table 1.