Fig 2.
Southern blot hybridization of D. melanogaster lines. Lane 1, initial untransformed strain y w67c23(2); lanes 2 and 3, lines transformed with construct B (containing the Penelope ORF under control of the D. melanogaster heat shock promoter); lanes 4–8, the progeny of one of five individual females taken from lines transformed with construct A (lanes 4 and 5 from line A1; lanes 6–8 from line A2). In all lanes, DNA was digested by XhoI and hybridized with the Penelope XhoI–XhoI 2.8-kb internal fragment.