Fig 2.
Analysis of T cell frequency in PBMCs after infusion of MART1-specific CD8+ T cell clones without IL-2. Two methods were used: semiquantitative PCR analysis of a clone-specific CDR3 region (gel bands digitized and quantified based on dilution standards 10−2 to 10−5) and tetramer analysis by flow cytometry. The T cell frequency in both cases was characterized by a rapid rise on day +1 after the infusion followed by a steady decline to nearly undetectable levels by day +14 and a median T cell survival in each case of ≈7 days.