Fig 4.

Induction of nonlymphoid FasL mRNA after transfer of TCR transgenic lymphocytes and antigen stimulation. (A) CD4⁺ lymphocytes from DO11.10 mice were introduced i.v. into B10.D2 recipient animals followed by antigenic stimulation (OVA 323–339 in CFA, s.c.). IECs and splenocytes were harvested 3 days postantigen. cDNA from each respective tissue was generated, and full-length FasL was amplified and digested with AluI to identify the source of FasL RNA. Splenocyte FasL was included from BALB/c, DO11.10, and B10.D2 to demonstrate each restriction-fragment pattern. (B) Further demonstration that induction of IEC FasL is nonlymphoid. B10.D2 mice were given SEB and lymphocytes isolated 3 days later and transferred into BALB/c-SCID recipients. Twenty-four hours after transfer, IECs were isolated and RNA was purified. Full-length cDNA was amplified and subjected to AluI restriction-digest analysis.