Fig 3.

8-Br-cGMP selectively enhanced type 1 cell differentiation. CD4⁺ T cells from D0.11 mice were negatively purified and cultured under Th1 or Th2 conditions as in Fig. 2 b and c. Graded concentrations of 8-Br-cGMP were added at the beginning of cultures, which were harvested on day 4. Results, representative of four similar experiments, are mean ± SD, n = 4, *, P < 0.05, compared with culture without 8-Br-cGMP. Similar results were obtained with polyclonally activated (anti-CD3 antibody-treated BALB/c CD4⁺ T cells) Th1 and Th2 cells and Tc1 and Tc2 cells (data not shown).