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Cerebral expression of IFN-regulated genes is compromised in GIFN12 STAT1-null mice. (A) RPA analysis for various signal-transduction and other IFN-regulated (B) genes in poly(A)+ RNA (1 μg per sample) extracted from the brain of 3-month-old mice. Samples in each lane are derived from individual animals. (C and D) Autoradiographs were quantified by densitometry, and the values were normalized to the housekeeping gene L32 by using NIH IMAGE V.1.57 software.
