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Activation of STAT 3 but not other signal-transduction molecules in the brain of GIFN12 STAT1-null mice. (A) Immunoblot analysis of STAT and various other signal-transduction molecules (B) in total protein lysates (100 μg per lane) from the basal forebrain of 3-month-old mice. (C) STAT3 activation in splenic leukocytes after treatment (40 min) with murine IFN-α (1,000 units/ml). Immunoblot analysis of total protein lysates (200 μg per lane) also is shown.
