Fig 5.

The bile-acid (a) and neutral sterol (b) content of feces from G5G8^−/− mice and their littermate controls and in vivo cholesterol synthesis (c). Feces were collected from individually housed 17-week-old mice of the indicated genotypes for 3 days while they consumed a chow diet. The daily excretion of total bile acids (a) and neutral sterols (b) was determined as described in Materials and Methods. (c) Four-month-old male and female wild-type (WT) and knockout (KO) mice (n = 6 in each group) maintained on a chow diet were injected i.p. with 40 mCi of [³H]H₂O (1 Ci = 37 GBq). The mice were killed 1 h later (mid-daylight cycle), and the tissues were collected and processed as described in Materials and Methods. +/+, wild-type mice; −/−, G5G8^−/− mice. *, P < 0.05 between wild-type and G5G8^−/− mice.