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Quantitative real-time PCR of liver RNA from wild-type and knockout mice. Total RNA from the livers of mice in each group (n = 5) was isolated and pooled for real-time PCR as described (31, 32). Cyclophilin was used as an internal control for these studies. Each value represents the mRNA level relative to the amount of transcript in the chow-fed wild-type males, which was set arbitrarily to 1. +/+, wild-type mice; −/−, G5G8−/− mice.
