Table 2.

Density of the ODN-probe 2 immobilized on the silicon surface (1.5 cm² exposed area) as function of its concentration in solution^a

ODN concentration in solution (µM)	ODN density on silicon surface (1012 strands/cm2)	Hybridization efficiency %b
27.135	8.10	40
13.567	9.44	73
2.714	6.54	92
0.543	5.64	100
0.135	2.09	100

^aReaction conditions: 4 ml phosphate buffer plus EDAC 0.026 M and NHS 0.0017 M; overnight; room temperature.

^bAfter hybridization, denaturation and fluorescence measurements in solution (see text).