Table 3.

Density of the ODN-target 3 hybridized^a with the ODN-probe 2 immobilized on the silicon surface of 1.5 cm² exposed area^b, as function of its concentration in solution

ODN concentration in solution (µM)	ODN density on silicon surface (1012 strands/cm2)
10.615	5.60
9.369	5.63
3.123	2.39
1.874	2.82
1.615	2.17
0.625	2.39
0.312	1.95

^aReaction conditions: 4 ml hybridization buffer, at 90°C for 5 min and then at 5°C for 1 h (see Materials and Method).

^bODN-probe 2 density on silicon surface for the various samples is nearly constant: 5.64–5.87 × 10¹² strands/cm².