Figure 2. Inhibition of FDC-P1 cell survival and proliferation in co-culture with AFT-024 cells in which ganglioside synthesis was inhibited by PDMP.

FDC-P1 cells washed previously with CMF-BSS were inoculated on to the semi-confluent monolayers of AFT-024 cells at 1×10⁵ cells/well and maintained for 0, 24 and 48 h. C, co-cultures maintained in standard conditions. PDMP, co-cultures maintained with 10 μM PDMP after inoculation of FDC-P1 cells upon AFT-024 semi-confluent cell monolayer treated previously with the inhibitor for 48 h. Phase-contrast microscopy of the cells is shown. Scale bar, 20 μm.