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Proteins were detected by Coomassie Brilliant Blue R-250 staining. Lane 1, molecular mass markers; lane 2, purified wild-type enzyme; lane 3, total cellular extract of non-transformed E. coli XL1Blue; lane 4, wild-type enzyme; lane 5, D155A; lane 6, D155G; lane7, T152A; lane 8, F151A; lane 9, A156G; lane 10, D155A/T152A; lane 11, F151A/A156G.
