Figure 6. Cellular expression of PTCH1 deletion constructs.

(A) Diagram representing the C-terminal deletion constructs of PTCH1 that were used in the present study. The end of each construct is indicated by the corresponding amino acid based on the numbering of GenBank® entry NM_000264. (B) COS7 cells transfected with PTCH1, PTCH1 Δ9–12, PTCH1 Δ8–12 or PTCH1 Δ2–12 constructs. The cells were stained with antibodies recognizing epitopes at the PTCH1 N-terminal domain. The proteins were visualized by immunofluorescence microscopy. The number of transfected cells and the amount of protein expressed in individual cells were comparable with each of the four constructs used. The cells were also stained with the nuclear marker DRAQ™.