Figure 8. Analysis of endogenous Gli1 inhibition by PTCH1 in Sufu^−/− cells.

(A) Sufu^−/− mouse embryonic fibroblast cells were transfected with PTCH2 reporter, Renilla luciferase transfection control and expression constructs as indicated. Note that PTCH1 inhibited the endogenous Gli1 activation of the reporter construct, while PTCH1 Δ2–12 did not. (B) As in (A), except that the reporter construct (8×GLIBS) consisted of eight copies of the GLI-binding consensus site linked to the luciferase gene.