Figure 2. Phosphate-transfer activities of the wild-type and D16E mutant LdAdK.
Under standard assay conditions, the rate of AMP formation by the wild-type (●, Wt) and D16E (○) enzymes were plotted against increasing concentrations of Ado (0.4 and 0.8 μg of enzyme was used respectively). Inset: specific activities of the respective enzymes, where the specific activity (Sp. Activity) of the D16E mutant was measured at 2 mM Ado. Results are means±S.D. for at least three experimental determinations.