Figure 2. Identification of peptide-displaying phages selected against Aedes aegypti BBMV that inhibit Cy11Aa binding to BBMV.

(A) Qualitative binding assay of biotinylated Cry11Aa toxin to BBMV in solution, performed in the presence of different phages (1×10¹¹ pfu) selected from panning against Ae. aegypti BBMV. The results are representative of three independent experiments. (B) Peptide-phage overlay assays to BBMV proteins. Lane 1, molecular mass marker; lanes 2, 3 and 4, binding of peptide-phage P1.BBMV and lanes 5, 6 and 7, binding of P8.BBMV, visualised with anti-M13 antibody. Lanes 2 and 5 correspond to total BBMV proteins. Lanes 3 and 6 to proteins recovered after PIPLC treatment of BBMV. Lanes 4 and 7 proteins recovered after Cry11Aa ligand chromatography.