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The values are means for three experiments and the standard deviations are indicated for UDP-Gal and UDP-Glc. The values for UDP-GalNAc and UDP-Glc are estimates only because of difficulties in the HPLC separation of the two compounds.
| Substrates | Km (μM) | kcat (s−1) | kcat/Km (s−1·μM−1) |
|---|---|---|---|
| UDP-Gal | 40±4 | 21±3 | 550±80 |
| UDP-Glc | 55±1 | 11±2 | 220±30 |
| UDP-GalNAc | 160 | 0.12 | 0.8 |
| UDP-GlcNAc | 200 | 0.07 | 0.4 |
