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F104X, F104W and V107X represent spectra of replacements of (A) Phe-104 with one of the amino acids in the present study, replacement of Phe-104 with Trp, and (B) replacements of Val-107 with one of the amino acids in the present study respectively. Fluorescence intensity was measured in arbitrary units. Additional lines illustrate variation between all the engineered GSTs.
