Table 3. Lock-and-key ‘Clasp’ residues in insect GST classes.
Numbering of residues is based on adGSTD4 sequence. *adGST, agGST and LcGST represent GSTs from A. dirus, A. gambiae and L. cuprina respectively. D is Delta, u is unclassified, E is Epsilon, T is Theta, S is Sigma, Z is Zeta and O is Omega GST class. GenBank® accession numbers: adGSTD1 (AF273041), adGSTD2 (AF273038), adGSTD3 (AF273039), adGSTD4 (AF273040), adGSTD5 (AF251478), adGSTD6 (AY014406), agGSTD1-3 (Protein ID AAC79992), agGSTD1-4 (Protein ID AAC79994), agGSTD1-5 (Protein ID AAC79993), agGSTD1-6 (Protein ID AAC79995), agGSTD2 (Z71480), agGSTD3 (AF513638), agGSTD4 (AF513635), agGSTD5 (AF513634), agGSTD6 (AF513636), agGSTD7 (AF071161), agGSTD8 (AF316637), agGSTD9 (AY255857), agGSTD10 (AF515527), agGSTD11 (AF513637), agGSTD12 (AF316638), agGSTu1 (AF515521), agGSTu2 (AF515523), agGSTu3 (AF515524), agGSTE1 (AF316635), agGSTE2 (AF316636), agGSTE3 (AY070234), agGSTE4 (AY070254), agGSTE5 (AY070255), agGSTE6 (AY070256), agGSTE7 (AF491816), agGSTE8 (AY070257), agGSTT1 (AF515526), agGSTT2 (AF515525), agGSTS1-1 (L07880), agGSTS1-2 (AF513639), agGSTZ1 (AF515522), agGSTO1 (AY255856) and LcGST (P42860). ‡104a and 104b indicate that the residue at this position is from different subunits. ‡agGSTD6 and agGSTD9 were suggested to be pseudogenes [6].
| Key residue | Lock residue | ||||||
|---|---|---|---|---|---|---|---|
| Enzyme* | 104a† | 65 | 67 | 68 | 103 | 104b† | 107 |
| adGSTD4 | F | E | R | A | L | F | V |
| adGSTD1 | F | E | R | A | L | F | M |
| adGSTD2 | Y | E | R | A | L | Y | M |
| adGSTD3 | Y | E | R | A | L | Y | M |
| adGSTD5 | H | E | R | V | L | H | L |
| adGSTD6 | F | E | Y | A | L | F | I |
| agGSTD1-3 | F | E | R | A | L | F | V |
| agGSTD1-4 | Y | E | R | A | L | Y | M |
| agGSTD1-5 | Y | E | R | A | M | Y | M |
| agGSTD1-6 | Y | E | R | A | L | Y | M |
| agGSTD2 | F | E | R | A | L | F | A |
| agGSTD3 | F | E | Y | A | L | F | I |
| agGSTD4 | F | E | C | A | L | F | V |
| agGSTD5 | F | E | Y | A | L | F | I |
| agGSTD6‡ | F | E | S | A | L | F | I |
| agGSTD7 | H | E | R | V | L | H | L |
| agGSTD8 | F | E | R | A | L | F | H |
| agGSTD9‡ | L | E | G | A | L | L | C |
| agGSTD10 | F | E | Y | A | L | F | N |
| agGSTD11 | Y | E | R | A | L | Y | M |
| agGSTD12 | F | E | Y | A | L | F | S |
| agGSTu1 | C | E | N | A | L | C | L |
| agGSTu2 | H | E | R | A | L | H | L |
| agGSTu3 | Y | E | K | A | L | Y | S |
| agGSTE1 | H | E | H | A | L | H | S |
| agGSTE2 | H | E | H | A | L | H | S |
| agGSTE3 | H | D | H | A | L | H | S |
| agGSTE4 | H | D | H | A | L | H | S |
| agGSTE5 | H | D | H | A | L | H | S |
| agGSTE7 | H | A | H | A | L | H | A |
| agGSTE6 | F | D | H | A | L | F | S |
| agGSTE8 | C | D | H | A | L | C | N |
| agGSTT1 | E | E | V | A | L | E | H |
| agGSTT2 | S | E | V | A | L | S | H |
| agGSTS1-1 | D | Q | L | A | V | D | N |
| agGSTS1-2 | D | Q | V | A | V | D | N |
| agGSTZ1 | E | E | V | S | C | E | A |
| agGSTO1 | E | E | L | V | I | E | A |
| LcGST | Y | E | R | A | L | Y | M |