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. 2006 Jan 27;394(Pt 1):173–183. doi: 10.1042/BJ20051296

Figure 2. DMT1 protein down-regulation is earlier than mRNA down-regulation.

Figure 2

(A) Western-blots for DMT1 isoforms using cell lysates during the first 24 h after removal of RA. Cells were collected at 0, 2, 4, 10 and 24 h after replating without RA and Western-blot showed that +IRE and −IRE isoforms were decreased after 4 h, while the 1A isoform did not show any significant change. (B) RT-PCR for DMT1 isoforms during the first 24 h after removal of RA. Total RNA was collected at 0, 4, 10 and 24 h after replating without RA, and RT-PCR was performed as described. The results showed that+and−isoforms were decreased after 10 h, while there was no change in the 1A mRNA isoform. UD, undifferentiated cells.