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. 2006 Jan 27;394(Pt 1):173–183. doi: 10.1042/BJ20051296

Figure 6. Nitric oxide production is responsible for the down-regulation of 1B DMT1.

Figure 6

(A) RT-PCR was done using total RNA from undifferentiated cells (UD), cells 18 h after removal of RA (day5) and cells after removal of RA treated with 500 μM L-NMMA (NMMA) for 18 h. The results showed that the expression of the 1B isoform was rescued in cells treated with the NOS inhibitor L-NMMA. No change was seen in 1A isoform mRNA. (B) RT-PCR was done using total RNA from cells during differentiation. The results show increased expression of neuronal NOS 10 h after removal of RA.