Figure 10. Blocking of the 15d-PGJ₂-dependent potentiation of the PT by NEM.

NEM (50 μM) was added to mitochondria (1 mg/ml) in swelling buffer and allowed to incubate for 2 min. (A) 15d-PGJ₂ (20 μM) was added after the NEM and allowed to react for an additional 2 min before the addition of Ca²⁺ (130 μM). Lag time (after the addition of Ca²⁺) was calculated as described in Figure 1. Data are single traces representative of three individual experiments. (B) The lag time before onset of swelling is indicated by the histogram. Results are expressed as the means±S.E.M. for at least three independent experiments. *, P<0.05 for Ca²⁺+15d-PGJ₂-treated mitochondria compared with Ca²⁺-treated.