Figure 2. Promotion of cytochrome c release from isolated mitochondria by 15d-PGJ₂.

Mitochondria were incubated with and without 15d-PGJ₂ (10 μM), 2 min after which Ca²⁺ was added. Cyclosporin A (1 μM) and EGTA (20 μM) were added at 1 min to stop further pore opening and cytochrome c release, and mitochondria were separated by centrifugation for measurement of cytochrome c in the supernatant. (A) Representative HPLC trace for mitochondria that were untreated or treated with Ca²⁺ alone, or Ca²⁺ and 15d-PGJ₂. (B) Quantitation of cytochrome c released at 1 min expressed as fold change of the concentration of cytochrome c at time 0. Results are expressed as the means±S.E.M. for at least three experiments. *, P<0.001; #, P<0.05.