Figure 5. Ca²⁺-induced PT is not altered by the addition of the superoxide dismutase mimetic, MnTBAP.

Mitochondria (1 mg/ml) were incubated in swelling buffer with succinate (5 mM) and rotenone (1 μM). (A) Ethanol or 15d-PGJ₂ (20 μM) was added just prior to MnTBAP (50 μM) and allowed to incubate for 2 min before the addition of Ca²⁺ (130 μM). Lag time (after the addition of Ca²⁺) was calculated as described in Figure 1. Data are single traces representative of three individual experiments. (B) The lag time before onset of swelling is indicated in the histogram. Results are expressed as the means±S.E.M. for at least three independent experiments. *, P<0.05 for Ca²⁺+15d-PGJ₂-treated mitochondria compared with Ca²⁺-treated. #, P<0.05 for Ca²⁺+MnTBAP+15d-PGJ₂-treated mitochondria compared with Ca²⁺+MnTBAP-treated.