Figure 9. Modification of mitochondrial proteins by bt-15d-PGJ₂ is blocked by NEM.

(A) Mitochondria (1 mg/ml) in respiration buffer were incubated with NEM (0, 50 or 500 μM) for 10 min, followed by treatment with or without bt-15d-PGJ₂ (50 μM) for 1 h. Protein adducts were detected by Western blot analysis. The asterisks mark the biotin-containing mitochondrial carboxylases. (B) Quantitation of density from (A) expressed as normalized blot density (total density of lane/density of carboxylase bands in that lane). Results are expressed as the means±S.E.M. (n=3). *, P<0.01 versus bt-15d-PGJ₂ only.