Table 1. Affinity-precipitated proteins from liver mitochondria following treatment with bt-15dPGJ2.
Proteins identified by MS as being associated with modification by bt-15d-PGJ2 are listed together with their predicted molecular mass and MOWSE (molecular weight search) score. The first column identifies the spot or band number for each protein as indicated in Figure 11. The values for each MOWSE score are given in the order of the spot number. The accession number is reported in the final column. The numbers in the first column can be cross-referenced to the band numbers or spots in Figures 11(A) and 11(C).
| Band/spot no. | Protein | Predicted mass (kDa) | MOWSE score | Accession no. |
|---|---|---|---|---|
| 1, 25, 26 | Carbamoyl-phosphate synthetase 1 | 164.4 | 180, 105, 73 | NP_058768 |
| 2, 3, 27 | Pyruvate carboxylase | 129.7 | 235, 173, 109 | NP_036876 |
| 4, 7 | Similar to RIKEN cDNA D330038I09 (predicted) | 96.9 | 207, 112 | XP_235005 |
| 5 | Hydroxyacyl-CoA dehydrogenase | 82.4 | 147 | NP_570839 |
| 6 | ANT | 74.2 | 153 | NP_056644 |
| 8 | Glutamate dehydrogenase 1 | 61.3 | 69 | NP_036702 |
| 9 | Heat-shock protein 1 (chaperonin) | 60.9 | 140 | NP_071565 |
| 10 | 3-Hydroxy-3-methylglutaryl-CoA synthase 2 | 56.9 | 110 | NP_775117 |
| 11 | Acetyl-CoA acyltransferase 2 | 41.8 | 158 | NP_569117 |
| 12 | 3-Hydroxybutyrate dehydrogenase | 38.3 | 159 | NP_446447 |
| 13 | Solute carrier family 25, member 5 | 32.9 | 107 | NP_476443 |
| 14 | 3-Hydroxyacyl-CoA dehydrogenase type II | 27.2 | 122 | O70351 |
| 15 | Similar to aldo-keto reductase family 1, member B8 (predicted) | 29.6 | 82 | XP_220074 |
| 16 | Mitochondrial ATP synthase, O subunit | 23.3 | 89 | NP_620238 |
| 17 | Similar to mitochondrial inner membrane protein (mitofilin) (predicted) | 82.3 | 107 | XP_232055 |
| 18, 19 | Heat-shock protein 1 (chaperonin) | 60.9 | 91, 141 | NP_071565 |
| 20 | ATP synthase β subunit | 51.1 | 104 | AAB02288 |
| 21–23, 31–33 | Mitochondrial aldehyde dehydrogenase | 53.3 | 119, 103, 129, 111, 218, 116 | AAM94394 |
| 24 | ATP synthase, H+-transporting, mitochondrial F0 complex, subunit d | 18.7 | 89 | NP_062256 |
| 28 | Mortalin (MTHSP70) | 73.8 | 68 | P48721 |
| 29 | Heat-shock protein 1 (chaperonin) | 60.9 | 109 | NP_071565 |
| 30 | ATP synthase β subunit | 51.1 | 119 | AAB02288 |
| 34 | Propionyl-CoA carboxylase, β polypeptide | 58.6 | 127 | NP_058726 |
| 35–38 | Glutamate dehydrogenase 1 | 61.4 | 103, 145, 129, 127 | NP_036702 |
| 39 | Catalase | 59.7 | 132 | NP_036652 |
| 40, 44, 45 | ATP synthase α chain | 58.8 | 99, 128, 107 | P15999 |
| 41 | Methylcrotonoyl-CoA carboxylase 2 (β) (predicted) | 66.1 | 140 | NP_001012177 |
| 42–43 | Hydroxyacyl-CoA dehydrogenase | 82.5 | 129, 103 | NP_570839 |
| 46 | 3-Hydroxy-3-methylglutaryl-CoA synthase 2 | 56.9 | 169 | NP_775117 |
| 47 | Ubiquinol-cytochrome c reductase core protein II | 48.4 | 113 | NP_001006971 |