Table 1. Polar lipid metabolic labelling profiles in extracellular and intracellular Toxoplasma.
Lipid assessment was based on 2D-TLC and co-migration with characterized lipids from A. thaliana. Sphingolipids were identified by their resistance to alkaline hydrolysis with KOH in methanolic phase. U1–U8, unknown glycerolipids 1–8; (+), (++), (+++), qualitative detection scale; nd, not detected. In the case of U3–U7, occurrence in HFF cells was supposed from co-migrating spots in Toxoplasma samples.
| Toxoplasma tachyzoites | |||
|---|---|---|---|
| Lipid | Extracellular | Intracellular | Human HFF cells |
| Membrane glycerolipids | |||
| Ubiquitous phospholipids | |||
| PC | +++ | +++ | +++ |
| PE | +++ | ++ | +++ |
| PS | +++ | + | +++ |
| PI | + | + | ++ |
| PG | ++ | nd | ++ |
| Mitochondrial phospholipids | |||
| DPG | + | + | ++ |
| Chloroplast-like glycolipids | |||
| MGDG | nd | nd | nd |
| DGDG | + | nd | nd |
| SQDG | nd | nd | nd |
| Uncharacterized glycerolipids | |||
| U1 | ++ | nd | nd |
| U2 | ++ | + | nd |
| U3 (PA?) | ++ | + | + |
| U4 | + | nd | + |
| U5 | + | nd | + |
| U6 | + | nd | + |
| U7 | ++ | + | + |
| U8 | nd | + | nd |
| Membrane sphingolipids | |||
| GlcCer | + | + | ++ |
| LacCer | + | nd | + |
| TriHexCer | + | nd | + |
| Globoside | + | nd | + |