Table 1. Effects of Se deficiency on oxidant stress.

BAECs were cultured in F-12K medium supplemented with 5% (v/v) FBS in the presence and absence of Se (10 ng/ml). GPX activity in nmol of NADPH oxidized/min. TxrR activity in A₄₁₂ units×1000/min per mg of protein. Intracellular ROS production was measured by relative fluorescence of peroxide-sensitive DCFH-DA probe. 15-LOX-1 (15-HETE and 15-HPETE) metabolites in pmol/10⁶ BAECs per h following stimulation with 30 μM AA and 2 μM calcium ionophore A23187. Results are means±S.E.M. for three individual experiemts. *, P<0.05 compared with corresponding value in +Se group (paired Student's t test).

	Activity
Assay	+Se	−Se
GPX	32.0±4.9	6.3±1.4*
TxrR	100.1±2.2	47.3±7.1*
ROS	6.7±1.4	13.9±3.4*
15-HETE	519±27.2	254±15.7*
15-HPETE	239±19.4	60±17.6*