Figure 1. Deletion of LCB4 overcomes the growth defect of Δlcb2 cells cultured in the presence of SPH.

(A) TMY77 (Δlcb2) and TMY60 (Δlcb2 Δlcb4) cells were streaked on to YPD plates in the absence or presence of 5 μM PHS or SPH and incubated for 3 days at 30 °C. (B) TMY85 (Δlcb2 Δlcb4 Δsur2) cells were cultured in YPD medium containing 5 μM PHS overnight. The cells were washed, diluted (0.1 D₆₀₀ unit/ml) in YPD medium containing the indicated amounts of PHS, DHS or SPH and incubated at 30 °C. At the indicated times, the cell growth was determined by measuring the attenuance at 600 nm (D₆₀₀) using a spectrophotometer. Results shown are the average for two independent experiments.