Figure 5. Effect of Ca²⁺ or the antibiotic hygromycin B on the growth of mutants deficient in sphingolipids cultured with various LCBs.

TMY85 cells were cultured overnight in YPD medium containing 5 μM PHS. The cells were washed and diluted to 0.1 D₆₀₀ unit/ml in YPD medium with 5 μM PHS, DHS or SPH and cultured at 30 °C for an additional 24 h in the absence or presence of increasing concentrations of CaCl₂ (A) or hygromycin B (B). After a 24 h incubation, the cell growth was determined by measuring the attenuance at 600 nm (D₆₀₀) using a spectrophotometer. Results shown are the means±S.D. for three independent experiments.