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. 2006 Jan 27;394(Pt 1):243–248. doi: 10.1042/BJ20051635

Figure 2. Characterization of the initial light-dependent step of the reaction for MV and DV Pchlides.

Figure 2

The inset shows the 77 K fluorescence emission spectra of samples containing 1.1 μM DV Pchlide, 30 μM POR and 200 μM NADPH after illumination for 10 min at 130, 150, 165, 175 and 185 K. The intensity of the fluorescence band at 644 nm was measured at 77 K using an excitation wavelength of 450 nm. The temperature dependence of the initial photochemical step for MV and DV Pchlides, derived from these spectra, is shown. The decrease in fluorescence after illumination for 10 min at the respective temperatures was calculated relative to the largest decrease.