Figure 5. Lysosomal stabilization following exposure to Zn.

After incubation with 100 μM Zn for 1, 6, 12 or 24 h, cells were exposed to an initial bolus dose of 100 μM H₂O₂ for 30 min, followed by 6 h under standard culture conditions. AO staining and analysis by flow cytofluorimetry showed time-dependently increased lysosomal stability in cells incubated with Zn for 6 h or more. Results (percentage of cells with reduced number of intact lysosomes) are expressed as mean±S.D. and represent a minimum of four experiments. Comparisons were made with cells exposed to H₂O₂ (third bar). Examples of flow cytofluorimetry results are shown.