Figure 5. Induction of S. oneidensis SYE4 under different growth conditions.
The expression level of SYE4 was assayed with Western blot analysis after heat-shock treatment (temperature upshift from 28° to 42 °C) or after exposure to 2.5 mM paraquat, 0.2 mM H2O2, 0.05 mM CHP, 0.55 mM t-BOOH, 0.4 mM picric acid, 85 μM acrolein, 25 μM NEM or 10 mM 2-cyclohexen-1-one. Cells were harvested at the indicated time intervals and crude extracts were probed with antisera against SYE1. The position of the 37 kDa marker protein is indicated in the lane marked with an asterisk.