Fig 4.

Effect of gACRP30 on AMPK activity and related parameters in the incubated rat soleus. Muscles were incubated for 30 min in the presence (+) and absence (−) of gACRP30 (2.5 μg/ml). (A) Malonyl CoA concentration. (B) Uptake of 2-deoxyglucose. (C) AMPK activity. (D) Phosphorylation of AMPK and ACC and AMPK abundance. Similar differences between the soleus and EDL (see Fig. 1) have been observed when these muscles are incubated with the AMPK activator AICAR (20). See the legend to Fig. 1 for additional details. Blots are representative of muscles of two to five animals. Measurements of AMPK activity and malonyl CoA concentration are means ± SE (n = 5). *, P < 0.05 vs. gACRP30 (−) group.