Fig 5.

Effect of gACRP30 administered in vivo on AMPK activity and related parameters in mouse gastrocnemius muscle. Ten-week-old female C57BL/6J mice were fasted for 16 h, and then gACRP30 (75 μg per animal) (+) or saline [gACRP30 (−)] was injected into the retroorbital sinus. The rats were anesthetized with pentobarbital and muscle frozen in situ 15 or 30 min later. (A) AMPK activity (mean ± SE, n = 5) and malonyl CoA (n = 4). (B) Representative Western blots (n = 5) at 15 min. (C) Malonyl CoA concentration (mean ± SE, n = 5). (D) Representative Western blots of phosphorylated ACC at 30 min (n = 4). *, P < 0.05 vs. control.