Figure 5.

Lamin C is normally targeted to the nuclear envelope in Lmna^+/+ primary embryonic fibroblasts. Wild-type and Lmna^+/+ cells were incubated with antibodies against lamin A/C (red) and LAP2 (green) or DAPI to visualize DNA (blue), and the cells were examined by epifluorescence microscopy. The red fluorescence in wild-type cells identifies both lamin A and lamin C, whereas the red fluorescence in Lmna^+/+ cells represents only lamin C staining. Both lamin C and LAP2 in Lmna^+/+ cells were located in the nucleus and nuclear rim, and their distribution was indistinguishable from that of wild-type cells.