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(A) Absorption spectra of zeaxanthin in ethanol (1) and in detergent buffer used in reconstitution experiments (2). (B) 1, Absorption spectrum of PsbS reconstituted with zeaxanthin; 2, second derivative of spectrum 1; 3, CD spectrum of PsbS reconstituted with zeaxanthin; 4, simulated CD spectrum of two excitonically coupled zeaxanthin molecules with the higher and lower exciton components at 507 and 536 nm, respectively.
