Skip to main content
. 2006 Mar 1;116(3):675–682. doi: 10.1172/JCI27374

Figure 5.

Figure 5

Role of CaM and protein kinase in ET-1–induced HDAC5 nuclear export. (A) Myocytes were preincubated for 30 minutes with 4 μM K-252a (n = 8) or 20 μM W-7 (n = 7) followed by ET-1 (100 nM) application. (B) ET-1–induced nuclear export of HDAC5-GFP versus mutant nonphosphorylatable HDAC5 (HDAC5-S/A-GFP; n = 4). (C) Adv-GFP-HDAC5–infected myocytes were pretreated with 1 μM KN-93 (n = 6) or 10 μM Gö6976 (n = 8), separately or simultaneously for 20–30 minutes followed by ET-1 application. (D) Summary of HDAC5 nuclear export under different conditions. Elec stim, electrical stimulation; S/T PK, serine/threonine protein kinase; Tgc, transgenic.