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. 2002 Nov 25;99(25):16336–16341. doi: 10.1073/pnas.212624599

Fig 3.

Fig 3.

Expression of AGL24. (A–C) RT-PCR analysis of AGL24 expression. The β-tubulin gene (TUB2) was amplified as a quantitative control. (A) AGL24 expression in different organs. (B) Effect of photoperiod, GA, and vernalization on the expression of AGL24 and FLC. (C) AGL24 expression in late-flowering mutants in Columbia (Left) and Landsberg erecta (Right) backgrounds. The numbers indicate the relative expression levels of AGL24. (D) Northern blot analysis of AGL24 expression in transgenic plants. For 35S:AGL24 lines, total RNA was isolated from roots. 35S:AGL24 transgenic lines 3-6 and 8-4 represent weak and strong overexpression of AGL24, respectively. For AGL24 double-stranded interference (AGL24-RNAi) mutants, total RNA was isolated from stems. AGL24-RNAi 18-3 and 35-17 represent weak and strong mutant lines, respectively. The rRNAs stained by methylene blue indicate the amount of total RNA loaded in each lane.