Figure 2.
Fate of pro-CPY and invertase in sec13-1 mutant. (A, a) Sec13-1 cells (H230) were preincubated for 15 min at 37°C, 35S labeled for 5 min (lane 1), and chased with CHX at 37°C (lanes 2–5). The cell lysates were immunoprecipitated with anti-CPY antiserum followed by SDS-PAGE analysis. (A, b) The above-mentioned experiment was repeated at 24°C. Migration of ER (p1), Golgi (p2), and vacuolar (m) forms of CPY are indicated. (B) Sec13-1 cells (H230) were incubated in low-glucose medium (0.1%) at 24°C (squares) or 37°C (circles). Extracellular (EX, full symbols) and intracellular (IN, open symbols) invertase activity was determined and plotted against incubation time.