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Fate of Hsp150 in other COPII mutants. (A) Sec31-2 (H1102) and (B) Sec23-1 (H238) cells were preincubated for 15 min, 35S labeled for 5 min, and chased with CHX at 37°C. Cell lysate (c) and medium (m) samples were immunoprecipitated with Hsp150 antiserum before SDS-PAGE analysis. Mature and ER forms of Hsp150 are indicated on the right, and molecular weight markers (kilodaltons) on the left.
