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Secretion of β-lactamase activity. Sec13-1 cells (H1065) (A) and and control cells (H675) (B) grown at 24°C were shifted to fresh medium and incubated at 37°C. In A, CHX was added and the cells shifted to 24°C. Catalytic activity inside of the cells (open circles), in the cell wall (squares), and in the medium (closed circles) was determined and plotted against incubation time.
