Figure 8.
The Hsp150 fragment responsible for Sec13p-independent ER exit. Strains H1429 (SUI-Cterm/sec13-1) and H1508 (SUI-Cterm/control cells) (A) and H1067 (SUI-β-lactamase/sec13-1) (B) were preincubated for 15 min, 35S labeled for 5 min, and chased for 1 h (A), or as indicated (B). In B, lanes 11 and 12, sec18-1 (H840) and control cells (H839) were pulse labeled and chased. The medium (m) and cell lysate (c) samples were immunoprecipitated with Hsp150 antiserum (A) or β-lactamase antiserum (B) before SDS-PAGE analysis in 15% (A) or 8% (B) gels. The different forms of the reporter proteins are indicated on the right, and the molecular weight markers (kilodaltons) on the left.