Table 5.
Microtubule interactions with the cell cortex in bud6Δ cells
| Bud emergence | Spindle assembly | Spindle elongation
|
Spindle disassembly | ||
|---|---|---|---|---|---|
| Fast | Slow | ||||
| Bud cell cortex | |||||
| Time at cortex (%) | 21.3 | 42.0a | 36.9 | ||
| Number of events | 13 | 22 | 19 | 52 | 90 |
| Hit cortex (%) | 84.6 (45.1) | 91.0 (44.4) | 78.9 (61.7) | 34.6 (37.9) | 86.7 (43.1) |
| Grow at cortex (%)b | 7.7 (24.5) | 9.0 (5.0) | 5.2 (5.5) | 52.0c (22.5) | 8.9 (37.3) |
| Sweep at cortex (%) | 7.7 (9.5) | (17.5) | 15.8 (14.5) | 13.5 (16.4) | 4.4 (9.8) |
| Shrink at cortex (%)b | (20.8) | (33.1) | (18.3) | (23.2) | (9.8) |
| Duration of cortical contacts (min)d | 0.52 ± 0.1 | 0.57 ± 0.5a | 0.53 ± 0.1 | ||
| Mother cell cortex | |||||
| Time at cortex (%) | 31.5 | 38.9 | 57.0a | 41.8 | |
| Number of events | 34 | 55 | 13 | 44 | 58 |
| Hit cortex (%) | 88.0 (68.8) | 50.0 (42.5) | 53.8 (62.5) | 34.0 (13.5) | 77.6 (60.3) |
| Grow at cortex (%)b | 5.9 (12.5) | 32.0 (35.7) | 15.3 (12.5) | 25.0 (26.0) | 12.1 (20.2) |
| Sweep at cortex (%) | 5.9 (16.7) | 1.0 (1.5) | 11.3 (29.5) | 3.4 | |
| Shrink at cortex (%)b | (2.0) | 17.0 (20.3) | 30.8 (25.0) | 29.5 (31.0) | 6.9 (19.5) |
| Duration of cortical contacts (min)d | 0.54 ± 0.2 | 1.05 ± 0.46 | 1.68 ± 1.4a | 0.85 ± 0.4 | |
Numbers in small parentheses correspond to the same categories in wild-type cells provided for reference.
a
Data from fast and slow phase were combined.
b
Lines highlight the fact that modes of interaction associated with cortical Bud6p are absent in the bud of bud6Δ cells but are still present in the mother cell.
c
This category represents MTs growing along the cell cortex and was not accompanied by the SPB moving away from the cell cortex as in wild-type cells, in which MTs grew against the cortex.
d
Duration of cortical interactions represents the average of 20 independent events ± S.D.