Table 1.
Yeast two-hybrid analysis of interactions between mutant actins, wild-type actin, profilin, and cofilin
| Gal4-DBD-actin fusion | Interaction partner
|
|||
|---|---|---|---|---|
| Wild-type actin | Mutant actin | Profilin | Cofilin | |
| pGBT9 | − | na | − | − |
| Actin wt. | + | na | − | + |
| FL266/267GG | + | + | + | + |
| L267D | + | − | + | + |
| FL266/267DD | − | − | + | + |
| G13R | − | − | + | − |
| S14A | + | nd | − | + |
| S14C | + | nd | − | + |
| G15R | − | nd | + | + |
| V159N | + | nd | − | + |
For actin and profilin interactions, + indicates growth on plates containing 2 mM aminotriazole; for cofilin interactions, + indicates growth on plates without aminotriazole. No pGBT9 derivatives were sufficient for growth alone. na, not applicable; nd, not done.