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. 2002 Dec;13(12):4256–4265. doi: 10.1091/mbc.E02-06-0344

Figure 3.

Figure 3

Transcription analysis. (A) Northern blot of a 4% polyacrylamide-urea denaturing gel. A probe that hybridizes with lgl1 was used. Approximately 5 μg of the correspondent RNA was loaded in each well. The size of the transcripts is 867 bases, and the G418 concentration at which the transfected trophozoites were kept before lysis is 60 μg/ml unless otherwise stated. 1, pOV-LGL1; 2, strain HM1:IMSS; 3, pCYS-MUT; 4, pCONTROL; 5, pC-TRUNC (482 bases); 6, pDEEP-N (681 bases, 6 μg/ml G418); 7, strain HM1: IMSS; and 8, pN-TRUNC (702 bases, 6 μg/ml G418). (B) Relative quantity of transcripts as obtained by RT-PCR using the LightCycler. I, lgl1 and; II, lgl1 together with the 5′-truncated lgl1. 1 and 4, Nontransfected strain HM1:IMSS; 2 and 5, pCONTROL transfectant; 3 and 6, pN-TRUNC transfectant. Results represent the average of two independent experiments that gave very similar values.