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. 2002 Dec;13(12):4256–4265. doi: 10.1091/mbc.E02-06-0344

Figure 4.

Figure 4

Expression analysis. (A) Western blot of a 12% reducing SDS-PAGE probed with antilight subunit antibodies shows the expression of the light subunit genes/constructs in each of the transfectants. 1, pC-TRUNC; 2, pN-TRUNC; 3, pCONTROL; 4, Nontransfected HM1: IMSS strain; 5, pOV-LGL1; 6, pCYS-MUT; and 7, pDEEP-N. (B) Western blot of an 8% reducing SDS-PAGE probed with antilight subunit antibodies. 1, lysate of nontransfected HM1:IMSS; 2, lysate of transfectant pN-TRUNC showing the additional band at 32 kDa, which is detected above the 31-kDa band of nontransfected HM1:IMSS. (C) Western blot of a 10% reducing SDS-PAGE probed with antiheavy subunit antibodies showing the expression levels of the heavy subunit of the Gal-lectin in each of the transfectants. 1, Nontransfected HM1:IMSS; 2, pN-TRUNC; 3, pCONTROL; 4, pOV-LGL1; 5, pC-TRUNC; 6, pCYS-MUT; and 7, pDEEP-N.