Table 1.

Primers used in plasmid constructs

Primer	Primer sequence	Underlined restriction site
A	catgccatggttatattagtcttattgata	NcoI
B	gcgcgtcgacttatgcaaacacaggaataa	SalI
C	gctagagctcatgatgctatattttgttc	SacI
D	catgccatggttattgaatgttcaattcag	NcoI
E	gcgactagttgttttactcttagcacgaac	SpeI
F	gcgactagtagagttatttttgcatct	SpeI
G	ggagatctgaattgcataacttgtgtacat	BamHI
H	gcgcccatggttatataagtcttattgata	NcoI
I	aaagacttagcaccaaatgaataacttatc	DdeI
J	aaggtctcctaagtgtaaaacatgttgtag	BsaI and DdeI
K	aaggtctcaactctaccaaatgaataacttatc	BsaI
L	aaggtctcagagttatttttgcatctgatt	BsaI